ANG ( angiogenin , ribonuclease , RNase A family , 5 )

The amino acid sequence is available at NCBI protein locus AAA51678. It consists of a signal peptide from amino acid 1 to 24 and a mature peptide from amino acid 25 to 147. ANG is a basic, single chain potent blood-vessel inducing protein with a molecular weight of 14 kDa which was originally discovered in conditioned media of a human colon carcinoma cell line HT-29. ANG belongs to the RNAse superfamily, being 35% identical and 68% homologous to the pancreatic RNAse A sequence. The overall crystal structure of ANG shows a similarity to, but the biological actions of ANG differ distinctly from those of RNAse A. ANG possesses two distinct regions: a ribonucleolytic and a noncatalytic site, both being critical for angiogenic activity. Besides the ribonucleolytic activity, ANG differs from RNAse A in noncatalytic activities such as interactions with endothelial and smooth muscle cells and subsequent cellular responses in the events of neovascularization, including basement membrane degradation, signal transduction, and nuclear translocation.